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Proteases, the largest enzyme family encoded by the human genome, play a pivotal role in various physiological and pathological processes. From regulating cell proliferation and embryonic development to tissue remodeling and protein maturation, proteases are integral to numerous signaling pathways and represent promising drug targets for a range of diseases, including cardiovascular disorders and cancer. With the rise of protease inhibitors, particularly in the treatment of HIV and Hepatitis C, the need for comprehensive protease screening has never been more critical.
Understanding the Role of Proteases
Proteases are enzymes that catalyze the hydrolysis of peptide bonds in proteins, thereby regulating proteolytic mechanisms fundamental to many biological functions. They are implicated in various critical processes, including:
- Cell Proliferation and Development: Proteases are essential for embryonic development and the remodeling of tissues.
- Protein Activation and Maturation: Many proteins require proteolytic cleavage for their activation.
- Pathological Implications: Dysregulation of proteases has been associated with numerous diseases, making them significant therapeutic targets. For instance, eight approved HIV compounds belong to the class of protease inhibitors, and research continues into their implications for other conditions such as type II diabetes and Alzheimer’s disease.
Creative Bioarray’s Protease Screening Services
At Creative Bioarray, we offer a robust suite of protease screening services designed to facilitate drug discovery processes. Our extensive profiling and screening capabilities provide researchers with access to over 50 types of proteases, including cysteine, metalloproteinases, serine, threonine, and aspartic families. This flexibility is essential for discovering potential drug inhibitors targeting various protease enzymes.
Services Offered
- High Throughput Screening: Efficiently screen large libraries for potential drug candidates.
- Dose-Response Profiling: Conduct IC50 profiling with multiple dosing to determine compound efficacy.
- Protease Substrate Determination: Utilize peptide libraries for identifying appropriate substrates.
- Custom Assay Development: Tailored solutions to meet specific research needs.
Targeted Protease Families
- Cysteine Proteases: Includes Caspases (1-10), Cathepsins (B, H, K, L, S, V), and Calpain.
- Metalloproteases: Covers a wide range including ACE, MMPs (1-14), and Neprilysin.
- Serine Proteases: Targets like Thrombin, Factor Xa, and various DPP enzymes.
- Aspartate Proteases: Includes HIV-1 protease, Pepsin, and Renin.
Key Advantages
- High Sensitivity and Reproducibility: Our assays are designed to produce reliable data with minimal variation.
- Minimal Sample Requirement: Only nanoliters of compound are needed to perform assays.
- Rapid Data Delivery: Receive actionable insights within days of compound submission.
- 24/7 Support: Our team is available around the clock to guide you through your project and address any inquiries.
Conclusion
Creative Bioarray is dedicated to providing high-quality protease screening services that can significantly accelerate your drug discovery programs. With our state-of-the-art facilities, experienced team, and commitment to fast turnaround times, we are prepared to support your research needs effectively. Whether you’re involved in basic research or new therapeutic developments, our services are tailored to enhance your project outcomes.