Koaleszenz
In the dynamic landscape of drug discovery, the need for innovative screening methods has never been greater. High Content Screening (HCS), also known as automated microscope-based screening, represents one of the most advanced techniques available. HCS allows researchers to measure the biological activity of individual cells or whole organisms after treatment with various agents, employing cutting-edge microscope technology to gather critical data with unmatched precision.
What is High Content Screening?
High Content Screening involves the use of fluorescent dyes to characterize cells or organisms, enabling scientists to observe cellular responses to treatments across thousands of agents within multi-well plates. This multi-parametric approach provides extensive data on the composite phenotypes of cells, making HCS a powerful tool for assessing the effects of gene modifications or drug exposures concurrently.
As an analysis platform, HCS has rapidly gained traction across all stages of drug discovery processes. Its versatility allows for applications in hit-to-lead processes, mode-of-action studies during secondary and tertiary screenings, and even primary screenings to identify promising compounds early on.
Why Choose Creative Bioarray for High Content Screening?
At Creative Bioarray, we specialize in HCS services that encompass a comprehensive range of assay development, screening, and data analysis. Our state-of-the-art high-throughput microscopy systems yield high-quality images that serve as the foundation for our analyses. Our team’s expertise spans all facets of HCS, including:
- Sample Preparation: Ensuring optimal conditions for accurate measurements.
- Image Acquisition: Using advanced imaging techniques to capture the required data.
- Image Analysis: Employing sophisticated algorithms to interpret the data.
- Data Management: Effectively managing large volumes of data for easy retrieval and analysis.
Custom High Content Screening Assays
With over 200 available primary and stem cell-based HCS assays, Creative Bioarray offers flexibility in assay customization to meet specific research requirements. Some key functional assays we conduct include:
- Cellular Proliferation: Assessing the growth rates of cells under various conditions.
- Cell Apoptosis and Necrosis: Studying the processes of cell death and survival.
- Cytotoxicity: Measuring the toxic effects of compounds on cells.
- Cell Migration: Investigating movement patterns of cells in response to stimuli.
- Differentiation Markers: Identifying changes in cell type and function.
Moreover, we also explore complex biological phenomena such as mitochondrial physiology, inflammasome activation, and synaptic plasticity, among others.
Key Features of Our HCS Services
- Extensive Assay Options: With a library of over 200 assays, many can be customized.
- Collaborative Development: We work closely with clients throughout the assay development and data analysis processes.
- Advanced Models: Focused on utilizing inducible pluripotent stem (iPS) cells for high-quality data.
- Quality and Relevance: Committed to providing data that is both high quality and biologically relevant.
The Future of Drug Discovery with High Content Screening
As we advance our capabilities at Creative Bioarray, our focus remains on integrating HCS with high-throughput screening to facilitate the transition from traditional biochemical assays to dynamic phenotypic and genomic approaches. This allows us not only to study disease targets more effectively but also to innovate in the development of therapeutic molecules.
By leveraging HCS, researchers can gain insights into compound actions on various known targets, while simultaneously detecting potential side effects. This selective approach significantly increases the chances of successful drug development.
For those interested in comprehensive HCS services, Creative Bioarray is equipped to support your research needs. With customer service representatives available 24/7, we ensure that you have the assistance you require at every stage of your project.